線性PEI轉(zhuǎn)染試劑（速溶型）MW40000

英文名        Polyethylenimine Linear(PEI) MW40000（rapid lysis）

貨號(hào)           JX24765

保存條件    室溫

    線性PEI轉(zhuǎn)染試劑是一種陽(yáng)離子聚合物，分子量40000，它能與核酸形成復(fù)合物，并使該復(fù)合物進(jìn)入哺乳動(dòng)物細(xì)胞。線性PEI轉(zhuǎn)染試劑廣泛適用于常見細(xì)胞系，如 HEK-293、HEK293T、 Hep G2、Hela、CHO-K1、COS-1、COS-7、NIH/3T3 和 Sf9 等。該試劑即使在有血清存在的情況下，它仍然能高效的將核酸導(dǎo)入細(xì)胞。我公司提供的線性PEI轉(zhuǎn)染試劑具有如下優(yōu)點(diǎn)：優(yōu)越的轉(zhuǎn)染效率 , 重組蛋白的高表達(dá)水平,與含血清的培養(yǎng)基相兼容, 低細(xì)胞毒性,易于操作。

    本產(chǎn)品為速溶型，溶解迅速，配制方便。

說明書

 Transfection Reagent Preparation and Storage Recommendations

 Transfection Reagent Preparation (1 mg/mL)

 Materials:

   1.1g PEI 25K (kingmorn Catalog# KE1075).

   2.1L Milli-Q® water, water for injection (WFI), or comparable biological-grade water

   3.12 M Hydrochloric Acid (HCl)

   4.10 M Sodium Hydroxide (NaOH)

   5.Disposable 0.1-0.2um PES vacuum sterile-filter.

   6.Sterile HDPE or polypropylene storage vials for reagent aliquots.

Equipment

   1.1L glass beaker

   2.1L glass graduated cylinder

   3.Calibrated pH meter

   4.2 x disposable 1mL plastic pipettes

   5.Stir plate

   6.PTFE coated stir bar

   7.Vacuum pump

 Method:

   1.Dispense 1g of PEI 25K into beaker and suspend in 900 mL of water.

   2.Add stir bar and set stirring to produce a small vortex.

   3.Add hydrochloric acid dropwise until pH is < 2.0.

   4.Cover top of beaker and stir for up to 3 hours until powder fully dissolves.

   5.Add sodium hydroxide dropwise until pH is 6.9-7.1.

   6.Transfer solution to graduated cylinder and add water until the total volume is 1L.

   7.Sterile-filter through vacuum membrane.

   8.Aliquot as desired and store at -20℃.

 Reagent Storage

   Frozen aliquots can be stored at -20?C for up to one year. Aliquots can be thawed and kept at 4?C for up to two weeks, but should never be re-frozen.

 References

   1. Polyethylenimine (PEI), linear (1 mg/mL). Cold Spring Harbor Protocols 2008, pdb.rec11323– pdb.rec11323 (2008). doi:10.1101/pdb.rec11323